Hotspot

petD

LSC · 81461-82698 · 1238 bp

Back to Lafoensia

Pi 0.00485

MarkerSeek score 61.50

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1238
variable_sites: 6
pi: 0.00485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGTGAATTCTCCGAAGA	TCCCTAGATACACAAGTCGT	39.1	45.0	57.8	55.0	1320 bp	1.0	40.4
petD_p2	AGTTGAAGTGAATTCTCCGAAGA	GCGACTATTCCCTAGATACACA	39.1	45.5	57.8	56.9	1328 bp	1.0	40.4
petD_p3	AGTTGAAGTGAATTCTCCGAAGA	TTCCCTAGATACACAAGTCGT	39.1	42.9	57.8	55.8	1321 bp	1.0	40.4
petD_p4	AGTTGAAGTGAATTCTCCGAAGA	AGCGACTATTCCCTAGATACACA	39.1	43.5	57.8	58.3	1329 bp	1.0	40.4
petD_p5	GTTGAAGTGAATTCTCCGAAGAG	TCCCTAGATACACAAGTCGT	43.5	45.0	57.5	55.0	1319 bp	1.0	40.4