Hotspot

ccsA-ndhD

SSC · 120346-120714 · 369 bp

Back to Lafoensia

Pi 0.0352
MarkerSeek score 63.70
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
369
variable_sites
13
pi
0.0352
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGATATGCTATTTTGGGGT TCAATTGACAAGGTGGAAGC 40.0 45.0 54.0 56.2 489 bp 1.0 42.1
ccsA-ndhD_p2 TTGGATATGCTATTTTGGGGT TCAATTGACAAGGTGGAAGC 38.1 45.0 54.9 56.2 490 bp 1.0 42.1
ccsA-ndhD_p3 TTTGGATATGCTATTTTGGGGT TCAATTGACAAGGTGGAAGC 36.4 45.0 55.7 56.2 491 bp 1.0 42.1
ccsA-ndhD_p4 CATTTGGATATGCTATTTTGGGGT TCAATTGACAAGGTGGAAGC 37.5 45.0 57.6 56.2 493 bp 1.0 42.1
ccsA-ndhD_p5 TGGATATGCTATTTTGGGGT ATCAATTGACAAGGTGGAAGC 40.0 42.9 54.0 56.8 490 bp 1.0 42.1