Hotspot

rpl32-trnL-UAG

SSC · 118176-119205 · 1030 bp

Back to Lafoensia

Pi 0.0156

MarkerSeek score 53.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1030
variable_sites: 16
pi: 0.0156
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGGCGTTGAAAGCTTTTTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	1149-1154 bp	1.0	80.1
rpl32-trnL-UAG_p2	CGGCGTTGAAAGCTTTTTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	1150-1155 bp	1.0	80.1
rpl32-trnL-UAG_p3	CGGCGTTGAAAGCTTTTTCA	GGCGCAGTTATCACCCTTAA	45.0	50.0	58.2	58.0	1613-1616 bp	1.0	79.8
rpl32-trnL-UAG_p4	CGGCGTTGAAAGCTTTTTCA	TTATAAGACGCTATGCCGCC	45.0	50.0	58.2	57.9	1213-1218 bp	1.0	79.5
rpl32-trnL-UAG_p5	CGGCGTTGAAAGCTTTTTCA	TAGTTGGCGCAGTTATCACC	45.0	50.0	58.2	58.0	1621 bp	0.5	58.5