Hotspot

ndhF-rpl32

SSC · 117019-118001 · 983 bp

Back to Lafoensia

Pi 0.0163

MarkerSeek score 65.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 983
variable_sites: 16
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCACACACGACGGAAATTTT	TGAAAAAGCTTTCAACGCCG	45.0	45.0	57.2	58.2	1202-1206 bp	1.0	73.7
ndhF-rpl32_p2	TTGTTGCCGTTGGAAAAAGT	TGAAAAAGCTTTCAACGCCG	40.0	45.0	57.0	58.2	1182-1186 bp	1.0	71.6
ndhF-rpl32_p3	CCACACACGACGGAAATTTTT	TGAAAAAGCTTTCAACGCCG	42.9	45.0	57.9	58.2	1202-1206 bp	1.0	71.3
ndhF-rpl32_p4	TTTGTTGCCGTTGGAAAAAGT	TGAAAAAGCTTTCAACGCCG	38.1	45.0	57.7	58.2	1183-1187 bp	1.0	69.3
ndhF-rpl32_p5	TGTTGCCGTTGGAAAAAGTAG	TGAAAAAGCTTTCAACGCCG	42.9	45.0	57.3	58.2	1181-1185 bp	1.0	66.3