Hotspot

psbB-psbT

LSC · 78874-79067 · 194 bp

Back to Lafoensia

Pi 0.00515

MarkerSeek score 40.50

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 194
variable_sites: 1
pi: 0.00515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	ATTGTTAGAGCCGACGTTCC	TGAAGTAAGGAGCCTCCCAA	50.0	50.0	58.0	58.0	768-777 bp	1.0	81.1
psbB-psbT_p2	ATTGTTAGAGCCGACGTTCC	TTGAAGTAAGGAGCCTCCCA	50.0	50.0	58.0	58.0	769-778 bp	1.0	81.1
psbB-psbT_p3	ACGGAATTGTTAGAGCCGAC	AGTAAGGAGCCTCCCAACAT	50.0	50.0	58.0	58.0	770 bp	0.5	59.7
psbB-psbT_p4	ATTGTTAGAGCCGACGTTCC	AGTAAGGAGCCTCCCAACAT	50.0	50.0	58.0	58.0	765 bp	0.5	59.7
psbB-psbT_p5	GGAATTGTTAGAGCCGACGT	AGTAAGGAGCCTCCCAACAT	50.0	50.0	58.0	58.0	768 bp	0.5	59.7