Hotspot

petA-psbJ

LSC · 67329-68289 · 961 bp

Back to Lafoensia

Pi 0.00832

MarkerSeek score 63.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 961
variable_sites: 8
pi: 0.00832
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	ACTCCGGGTTAGGTTCATCC	50.0	55.0	58.0	58.8	1106-1107 bp	1.0	73.9
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	GGTTCATACTCCGGGTTAGG	50.0	55.0	58.0	57.1	1113-1114 bp	1.0	73.0
petA-psbJ_p3	GGCCTTTTGTTCTTCTTGGC	ACTCCGGGTTAGGTTCATCC	50.0	55.0	57.8	58.8	1089-1090 bp	1.0	73.0
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	ACTCCGGGTTAGGTTCATCC	50.0	55.0	58.2	58.8	1103-1104 bp	1.0	72.8
petA-psbJ_p5	GGCCTTTTGTTCTTCTTGGC	GGTTCATACTCCGGGTTAGG	50.0	55.0	57.8	57.1	1096-1097 bp	1.0	72.1