Hotspot

pafI-trnS-GGA

LSC · 47243-48090 · 848 bp

Back to Lafoensia

Pi 0.0059

MarkerSeek score 62.30

Primer available yes

Label pafI-trnS-GGA

Feature Metadata

label_name: pafI-trnS-GGA
feature_type: igs
region: LSC
length_bp: 848
variable_sites: 5
pi: 0.0059
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1010-1011 bp	1.0	67.0
pafI-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.4	58.1	1011-1012 bp	1.0	67.0
pafI-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	981-982 bp	1.0	66.3
pafI-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	TTCCAATGCTACGCCTTGAA	45.0	45.0	56.4	57.8	982-983 bp	1.0	66.3
pafI-trnS-GGA_p5	TCTCCTGAAGTTGTCGGAAT	CCCTCGGTAAACAAAAGCCT	45.0	50.0	56.2	58.1	1009-1010 bp	1.0	65.2