Hotspot

psaA-pafI

LSC · 44549-45244 · 696 bp

Back to Lafoensia

Pi 0.00583

MarkerSeek score 63.50

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 4
pi: 0.00583
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	TGAGAAATGACCCGGTTTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	884-893 bp	1.0	80.2
psaA-pafI_p2	GCCCATTCCTCGAAAGAAGT	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	865-874 bp	1.0	80.0
psaA-pafI_p3	TGAGAAATGACCCGGTTTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	886-895 bp	1.0	79.4
psaA-pafI_p4	GCCCATTCCTCGAAAGAAGT	CGATCAAGCCGCTGAGTATT	50.0	50.0	57.9	58.1	867-876 bp	1.0	79.2
psaA-pafI_p5	TGAGAAATGACCCGGTTTGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	880-889 bp	1.0	78.4