Hotspot

psbK-psbI

LSC · 8375-8758 · 384 bp

Back to Lafoensia

Pi 0.013

MarkerSeek score 61.70

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 384
variable_sites: 5
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	50.0	50.0	57.9	57.6	522-524 bp	1.0	77.1
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	519-521 bp	1.0	75.1
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	520-522 bp	1.0	75.1
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	521-523 bp	1.0	75.1
psbK-psbI_p5	TGTTTGGCAAGCTGCTGTAA	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.6	57.6	523-525 bp	1.0	72.8