Hotspot

ycf1

SSC · 123002-128203 · 5202 bp

Back to Lactuca

Pi 0.0221

MarkerSeek score 65.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5202
variable_sites: 656
pi: 0.0221
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCACGATACATCCCAGTCA	GGCCCTCCTATCTCTTCCTT	50.0	55.0	57.8	57.9	1217-1307 bp	1.0	88.4
ycf1_p2	TGCATCAGCGGAGAGTTTTT	GGCCCTCCTATCTCTTCCTT	45.0	55.0	58.1	57.9	1001-1058 bp	0.861111	82.2
ycf1_p3	TGCATCAGCGGAGAGTTTTT	TCGTTGTGGTCGGACTCTAT	45.0	50.0	58.1	58.2	1049-1106 bp	0.861111	81.6
ycf1_p4	TGCATCAGCGGAGAGTTTTT	ATCAATTCGGTCGTTGTGGT	45.0	45.0	58.1	57.8	1059-1116 bp	0.861111	81.5
ycf1_p5	TGCATCAGCGGAGAGTTTTT	CTAGCACTGGGTAGACCTCA	45.0	55.0	58.1	57.9	861-918 bp	0.861111	81.3