Hotspot

rpl32-trnL

SSC · 112563-113392 · 830 bp

Back to Lactuca

Pi 0.0204

MarkerSeek score 66.80

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 830
variable_sites: 120
pi: 0.0204
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	AAGGAAAGGATATTGGGCGG	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	939-1063 bp	1.0	89.0
rpl32-trnL_p2	AAGGAAAGGATATTGGGCGG	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	940-1064 bp	1.0	89.0
rpl32-trnL_p3	CGGCGTTAAAAGCTTTGTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.6	58.0	922-1046 bp	1.0	88.8
rpl32-trnL_p4	CGGCGTTAAAAGCTTTGTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.6	58.0	923-1047 bp	1.0	88.8
rpl32-trnL_p5	AAGGAAAGGATATTGGGCGG	AGCAAAGCCCTGGGTAAAAA	50.0	45.0	57.6	57.9	1443-1568 bp	1.0	88.3