Hotspot

ndhF-rpl32

SSC · 111348-112397 · 1050 bp

Back to Lactuca

Pi 0.0195

MarkerSeek score 75.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1050
variable_sites: 149
pi: 0.0195
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TGACAAAGCTTTTAACGCCG	50.0	45.0	58.0	57.6	1238-1344 bp	1.0	88.6
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	TGACAAAGCTTTTAACGCCG	45.0	45.0	58.0	57.6	1236-1342 bp	1.0	88.5
ndhF-rpl32_p3	GTTGCCGTCGGAAAAAGAAG	CCGCCCAATATCCTTTCCTT	50.0	50.0	58.0	57.6	1221-1327 bp	0.972222	87.7
ndhF-rpl32_p4	TGCCGTCGGAAAAAGAAGAA	CCGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	1219-1325 bp	0.972222	87.6
ndhF-rpl32_p5	TTGCCGTCGGAAAAAGAAGA	CCGCCCAATATCCTTTCCTT	45.0	50.0	58.0	57.6	1220-1326 bp	0.972222	87.6