Hotspot

rps18-rpl20

LSC · 68193-68440 · 248 bp

Back to Lactuca

Pi 0.0158

MarkerSeek score 67.10

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 248
variable_sites: 26
pi: 0.0158
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	AACCGAGTCGACCACTAGAA	AGACAGTTGCTTCTTAATCGT	50.0	38.1	58.1	55.3	406-423 bp	1.0	58.0
rps18-rpl20_p2	AAGAACCGAGTCGACCACTA	AGACAGTTGCTTCTTAATCGT	50.0	38.1	58.1	55.3	409-426 bp	1.0	58.0
rps18-rpl20_p3	TTGAAAGAACCGAGTCGACC	AGACAGTTGCTTCTTAATCGT	50.0	38.1	57.9	55.3	413-430 bp	1.0	57.5
rps18-rpl20_p4	CCGAGTCGACCACTAGAACT	AGACAGTTGCTTCTTAATCGT	55.0	38.1	58.5	55.3	404-421 bp	1.0	54.5
rps18-rpl20_p5	AGAACCGAGTCGACCACTAG	AGACAGTTGCTTCTTAATCGT	55.0	38.1	58.5	55.3	408-425 bp	1.0	54.4