Hotspot

trnF-ndhJ

LSC · 47918-48563 · 646 bp

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Pi 0.0132

MarkerSeek score 72.10

Primer available yes

Label trnF-ndhJ

Feature Metadata

label_name: trnF-ndhJ
feature_type: igs
region: LSC
length_bp: 646
variable_sites: 70
pi: 0.0132
unique_haplotype_count: 27
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACG	50.0	50.0	58.1	56.8	563-764 bp	1.0	81.4
trnF-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GGATAGGATGGCCTTTACGT	50.0	50.0	58.1	56.8	562-763 bp	1.0	81.3
trnF-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGATGGCC	50.0	47.6	58.1	57.7	570-771 bp	1.0	80.7
trnF-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTACG	50.0	47.6	58.1	57.5	564-765 bp	1.0	79.2
trnF-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TGGATAGGATGGCCTTTACGT	50.0	47.6	58.1	58.6	563-764 bp	1.0	78.4