Hotspot

trnE-UUC-rpoB

LSC · 12066-12996 · 931 bp

Back to Lactuca

Pi 0.0136
MarkerSeek score 71.70
Primer available yes
Label trnE-UUC-rpoB

Feature Metadata

label_name
trnE-UUC-rpoB
feature_type
igs
region
LSC
length_bp
931
variable_sites
83
pi
0.0136
unique_haplotype_count
32
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-rpoB_p1 GGGACTTGAACCCTCGTTTT AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.0 56.8 1023-1073 bp 1.0 72.5
trnE-UUC-rpoB_p2 TAACCACTGGACCATAGGGG AGTTCTTCTGTCAAACCCTGA 55.0 42.9 58.1 56.8 982-1032 bp 1.0 72.0
trnE-UUC-rpoB_p3 CCCTCGTTTTCTCCGTGAAA AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.1 56.8 1013-1063 bp 1.0 71.7
trnE-UUC-rpoB_p4 TTGAACCCTCGTTTTCTCCG AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.1 56.8 1018-1068 bp 1.0 71.7
trnE-UUC-rpoB_p5 GGGACTTGAACCCTCGTTTT TCAAACCCTGATCAATGAACCT 50.0 40.9 58.0 57.6 1013-1063 bp 1.0 71.1