Hotspot

trnC-GCA-petN

LSC · 9459-10267 · 809 bp

Back to Lactuca

Pi 0.0174

MarkerSeek score 70.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 809
variable_sites: 100
pi: 0.0174
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	863-893 bp	1.0	89.0
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	881-911 bp	1.0	86.7
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	862-892 bp	1.0	83.0
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	ATTAAAGCAGCCCAAGCGAG	50.0	50.0	58.1	58.9	871-901 bp	1.0	82.9
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	GACTACCATTAAAGCAGCCCA	50.0	47.6	58.1	58.0	878-908 bp	1.0	82.1