Hotspot

rps16-trnQ-UUG

LSC · 6303-7261 · 959 bp

Back to Lactuca

Pi 0.013
MarkerSeek score 66.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
959
variable_sites
102
pi
0.013
unique_haplotype_count
28
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1011-1046 bp 1.0 89.9
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1015-1050 bp 1.0 85.1
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1041-1076 bp 1.0 84.8
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1003-1038 bp 1.0 84.8
rps16-trnQ-UUG_p5 AGTCGCACGTTGCTTTCTAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.9 58.1 1010-1045 bp 1.0 84.1