Hotspot

trnK-UUU-rps16

LSC · 4374-5187 · 814 bp

Back to Lactuca

Pi 0.0169

MarkerSeek score 82.40

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 814
variable_sites: 90
pi: 0.0169
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GGTATGGATGAATCCCTCGC	TTCCTTGAAAAAGGCGCTCA	55.0	45.0	57.9	58.3	975-1042 bp	1.0	88.3
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	920-987 bp	1.0	88.0
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	58.2	58.3	919-986 bp	1.0	88.0
trnK-UUU-rps16_p4	TCAGTCGTGGTCTTCCAAAC	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1004-1071 bp	1.0	87.0
trnK-UUU-rps16_p5	CAGTCGTGGTCTTCCAAACT	TTCCTTGAAAAAGGCGCTCA	50.0	45.0	57.8	58.3	1003-1070 bp	1.0	87.0