Hotspot

ycf4

LSC · 58203-58757 · 555 bp

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Pi 0

MarkerSeek score 24.70

Primer available yes

Label ycf4

Feature Metadata

label_name: ycf4
feature_type: gene
region: LSC
length_bp: 555
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 24.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4_p1	TTGATTCAATCGTACCGCGT	CGTGTTTCCGGGTATTCCTT	45.0	50.0	58.0	57.9	1823-1831 bp	1.0	79.3
ycf4_p2	TTGATTCAATCGTACCGCGT	TTTGTAGACGTGTTTCCGGG	45.0	50.0	58.0	58.1	1831-1839 bp	1.0	79.1
ycf4_p3	GTGGGCCTAGTATTTCCAGC	CGTGTTTCCGGGTATTCCTT	55.0	50.0	58.0	57.9	1416-1426 bp	1.0	79.0
ycf4_p4	TTGATTCAATCGTACCGCGT	TCGTTGTGCGTCTTGATCAA	45.0	45.0	58.0	58.1	1886-1894 bp	1.0	79.0
ycf4_p5	TTGATTCAATCGTACCGCGT	TTCGTTGTGCGTCTTGATCA	45.0	45.0	58.0	58.1	1887-1895 bp	1.0	79.0