Pi
0.00163
MarkerSeek score
59.70
Primer available
yes
Label
rps4
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rps4_p1 | TGCTTGATTTATCATATTTGTCA | ACTAGAAAAAGTGGGCAAGGA | 26.1 | 42.9 | 52.5 | 57.1 | 794 bp | 1.0 | 40.1 |
| rps4_p2 | ACAATACTTTAGTAGATTTTGCTTGA | ACTAGAAAAAGTGGGCAAGGA | 26.9 | 42.9 | 55.5 | 57.1 | 813 bp | 1.0 | 40.1 |
| rps4_p3 | ACTTTAGTAGATTTTGCTTGATT | ACTAGAAAAAGTGGGCAAGGA | 26.1 | 42.9 | 52.4 | 57.1 | 808 bp | 1.0 | 40.1 |
| rps4_p4 | TTGCTTGATTTATCATATTTGTCA | ACTAGAAAAAGTGGGCAAGGA | 25.0 | 42.9 | 53.3 | 57.1 | 795 bp | 1.0 | 40.1 |
| rps4_p5 | TGCTTGATTTATCATATTTGTCA | ACTAGAAAAAGTGGGCAAGGAA | 26.1 | 40.9 | 52.5 | 57.8 | 794 bp | 1.0 | 40.1 |