Hotspot

rps16

LSC · 54713-55803 · 1091 bp

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Pi 0.000917

MarkerSeek score 59.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1091
variable_sites: 1
pi: 0.000917
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCAATGACTATTCACGATTTCA	TAGAACAAGAGAACAGGGGG	31.8	50.0	53.6	56.2	1198 bp	0.5	20.0
rps16_p2	ATCGAAGGAAAACTTAGAAGT	TAGAACAAGAGAACAGGGGG	33.3	50.0	52.5	56.2	1250 bp	0.5	20.0
rps16_p3	ATCAATGACTATTCACGATTTCA	TAGAACAAGAGAACAGGGGG	30.4	50.0	54.2	56.2	1199 bp	0.5	20.0
rps16_p4	TCAATGACTATTCACGATTTCAT	TAGAACAAGAGAACAGGGGG	30.4	50.0	54.2	56.2	1198 bp	0.5	20.0
rps16_p5	TCAATGACTATTCACGATTTCA	ATAGAACAAGAGAACAGGGGG	31.8	47.6	53.6	56.7	1199 bp	0.5	20.0