Hotspot

petA

LSC · 59969-60931 · 963 bp

Back to Laburnum

Pi 0.00104

MarkerSeek score 59.30

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	GGAACTAGGCCAGTGACCTA	AGAAGCACAAAGAAAGGGCT	55.0	45.0	58.1	57.6	1143 bp	1.0	76.0
petA_p2	GGAACTAGGCCAGTGACCTA	ATGCTGATAAATCCGCGGAC	55.0	50.0	58.1	58.5	1045 bp	1.0	75.1
petA_p3	AGCAGAATCATGGACAGGGA	AGAAGCACAAAGAAAGGGCT	50.0	45.0	58.4	57.6	1160 bp	1.0	73.8
petA_p4	GCAGAATCATGGACAGGGAA	AGAAGCACAAAGAAAGGGCT	50.0	45.0	57.6	57.6	1159 bp	1.0	73.7
petA_p5	AACTAGGCCAGTGACCTACC	AGAAGCACAAAGAAAGGGCT	55.0	45.0	58.4	57.6	1141 bp	1.0	73.7