Hotspot

atpI

LSC · 22839-23582 · 744 bp

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Pi 0.00134
MarkerSeek score 59.50
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
LSC
length_bp
744
variable_sites
1
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 AATACATTGCAGCGGGCTAA ACAAGTCAAAAAGGAGATGGTTG 45.0 39.1 57.9 57.5 868 bp 1.0 51.5
atpI_p2 ATACATTGCAGCGGGCTAAA ACAAGTCAAAAAGGAGATGGTTG 45.0 39.1 57.9 57.5 867 bp 1.0 51.5
atpI_p3 TGCAGCGGGCTAAACTAAAA ACAAGTCAAAAAGGAGATGGTTG 45.0 39.1 58.1 57.5 861 bp 1.0 51.1
atpI_p4 TTGCAGCGGGCTAAACTAAA ACAAGTCAAAAAGGAGATGGTTG 45.0 39.1 58.1 57.5 862 bp 1.0 51.1
atpI_p5 ATTGCAGCGGGCTAAACTAA ACAAGTCAAAAAGGAGATGGTTG 45.0 39.1 57.9 57.5 863 bp 1.0 50.9