Hotspot

trnL-CAA-ndhB

IRb · 93239-93815 · 577 bp

Back to Laburnum

Pi 0.00173

MarkerSeek score 59.30

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 577
variable_sites: 1
pi: 0.00173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GTCTCGCGTGTCTACCATTT	ACCGGGAATATCAATGAACCC	50.0	47.6	58.0	57.8	665 bp	1.0	70.3
trnL-CAA-ndhB_p2	CTCGCGTGTCTACCATTTCA	ACCGGGAATATCAATGAACCC	50.0	47.6	58.0	57.8	663 bp	1.0	70.3
trnL-CAA-ndhB_p3	TACCATTTCACCACCAAGGC	ACCGGGAATATCAATGAACCC	50.0	47.6	58.1	57.8	653 bp	1.0	69.7
trnL-CAA-ndhB_p4	GCGTGTCTACCATTTCACCA	ACCGGGAATATCAATGAACCC	50.0	47.6	58.2	57.8	660 bp	1.0	68.8
trnL-CAA-ndhB_p5	TCTCGCGTGTCTACCATTTC	ACCGGGAATATCAATGAACCC	50.0	47.6	57.7	57.8	664 bp	1.0	68.1