Hotspot

rpl20-rps12

LSC · 67468-68222 · 755 bp

Back to Laburnum

Pi 0.00265

MarkerSeek score 60.30

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 2
pi: 0.00265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTTTGTCCTCCGTTTCCGAG	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.1	57.8	882 bp	1.0	77.6
rpl20-rps12_p2	TTTTGTCCTCCGTTTCCGAG	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.1	57.7	887 bp	1.0	77.0
rpl20-rps12_p3	TTTTGTCCTCCGTTTCCGAG	AGGAACGTGTACTAGGGTGT	50.0	50.0	58.1	57.7	825 bp	1.0	76.8
rpl20-rps12_p4	TTTTTGTCCTCCGTTTCCGA	ACAGCCAATCCGAAATGTCA	45.0	45.0	57.7	57.8	883 bp	1.0	76.0
rpl20-rps12_p5	TTTTTGTCCTCCGTTTCCGA	ACAAGACAGCCAATCCGAAA	45.0	45.0	57.7	57.7	888 bp	1.0	75.4