Hotspot

rpl33-rps18

LSC · 66336-66551 · 216 bp

Back to Laburnum

Pi 0.00926

MarkerSeek score 57.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 216
variable_sites: 2
pi: 0.00926
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AGAATCGACACAATACGCCC	AATCAATTCGATCCCCCGAC	50.0	50.0	58.1	57.7	396 bp	1.0	77.8
rpl33-rps18_p2	GCCCAGTCGATTGGAATTGA	AATCAATTCGATCCCCCGAC	50.0	50.0	58.2	57.7	380 bp	1.0	76.4
rpl33-rps18_p3	AATCGACACAATACGCCCAG	AATCAATTCGATCCCCCGAC	50.0	50.0	58.3	57.7	394 bp	1.0	75.6
rpl33-rps18_p4	ACAATACGCCCAGTCGATTG	AATCAATTCGATCCCCCGAC	50.0	50.0	58.3	57.7	387 bp	1.0	75.6
rpl33-rps18_p5	CACAATACGCCCAGTCGATT	AATCAATTCGATCCCCCGAC	50.0	50.0	58.3	57.7	388 bp	1.0	75.6