Hotspot

psbZ-trnG-GCC

LSC · 43888-44245 · 358 bp

Back to Laburnum

Pi 0.00559

MarkerSeek score 56.90

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 358
variable_sites: 2
pi: 0.00559
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CGGATGGTTGGTCGAGTAAC	CGTCTTCTCCTTGGCAAAGA	55.0	50.0	58.4	57.8	506 bp	1.0	76.1
psbZ-trnG-GCC_p2	TTTGCTTCTCCGGATGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.5	57.8	516 bp	1.0	75.2
psbZ-trnG-GCC_p3	ATTTGCTTCTCCGGATGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.5	57.8	517 bp	1.0	74.8
psbZ-trnG-GCC_p4	GGATGGTTGGTCGAGTAACA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.2	57.8	505 bp	1.0	72.9
psbZ-trnG-GCC_p5	GGATGGTTGGTCGAGTAACAA	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	505 bp	1.0	70.6