Hotspot

atpH-atpI

LSC · 21519-22838 · 1320 bp

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Pi 0.000758

MarkerSeek score 59.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1320
variable_sites: 1
pi: 0.000758
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CTCTACAGCTTGTCCTGCAG	CCGGTCATGTTCCTTGGATT	55.0	50.0	58.0	57.9	1532-1533 bp	1.0	79.0
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CCGGTCATGTTCCTTGGATT	45.0	50.0	58.0	57.9	1453-1454 bp	1.0	78.8
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCA	CCGGTCATGTTCCTTGGATT	50.0	50.0	57.9	57.9	1491-1492 bp	1.0	78.0
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CCGGTCATGTTCCTTGGATT	45.0	50.0	58.2	57.9	1454-1455 bp	1.0	77.6
atpH-atpI_p5	CTCTACAGCTTGTCCTGCAG	TACCGGTCATGTTCCTTGGA	55.0	50.0	58.0	58.4	1534-1535 bp	1.0	77.2