Hotspot

ndhJ-trnF-GAA

LSC · 12516-13120 · 605 bp

Back to Laburnum

Pi 0.00833

MarkerSeek score 64.30

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 605
variable_sites: 5
pi: 0.00833
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	TTGGATAGGATGGCCTTTGC	GGGATAGCTCAGCTGGTAGA	50.0	55.0	57.9	58.0	741-746 bp	1.0	80.5
ndhJ-trnF-GAA_p2	TTGGATAGGATGGCCTTTGC	CCTCGTGTCACCAGTTCAAA	50.0	50.0	57.9	58.1	705-710 bp	1.0	80.1
ndhJ-trnF-GAA_p3	TTGGATAGGATGGCCTTTGC	TCAAATCTGGTTCCTGGCAC	50.0	50.0	57.9	58.1	690-695 bp	1.0	79.9
ndhJ-trnF-GAA_p4	TTGGATAGGATGGCCTTTGC	AGAATGGTCGGGATAGCTCA	50.0	50.0	57.9	57.9	750-755 bp	1.0	79.8
ndhJ-trnF-GAA_p5	TTGGATAGGATGGCCTTTGC	CCAAGAATGGTCGGGATAGC	50.0	55.0	57.9	58.1	753-758 bp	1.0	79.6