Hotspot

trnK-UUU-rbcL

LSC · 4417-5224 · 808 bp

Back to Laburnum

Pi 0.00124

MarkerSeek score 59.50

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 808
variable_sites: 1
pi: 0.00124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AAAGCCGAGTACTCTACCGT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.2	58.0	917 bp	1.0	78.5
trnK-UUU-rbcL_p2	AAGCCGAGTACTCTACCGTT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.2	58.0	916 bp	1.0	78.5
trnK-UUU-rbcL_p3	AAAGCCGAGTACTCTACCGT	GAGGCTTCCAAATGGAGTCC	50.0	55.0	58.2	58.2	932 bp	1.0	76.8
trnK-UUU-rbcL_p4	AAGCCGAGTACTCTACCGTT	GAGGCTTCCAAATGGAGTCC	50.0	55.0	58.2	58.2	931 bp	1.0	76.8
trnK-UUU-rbcL_p5	TACCGTTGAGTTAGCAACCC	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.5	58.0	903 bp	1.0	76.1