Hotspot

trnH-GUG-psbA

LSC · 133-399 · 267 bp

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Pi 0.015
MarkerSeek score 61.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
267
variable_sites
4
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 335-336 bp 1.0 79.6
trnH-GUG-psbA_p2 TTCAATTTCTGGGCGAACGA TTCCCTCTAGACCTAGCTGC 45.0 55.0 58.1 57.9 399-400 bp 1.0 79.5
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 349-350 bp 1.0 79.5
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 348-349 bp 1.0 79.5
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC TTCCCTCTAGACCTAGCTGC 50.0 55.0 57.9 57.9 350-351 bp 1.0 79.5