Hotspot

rpl2

LSC · 632-1456 · 825 bp

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Pi NA

MarkerSeek score 46.20

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: LSC
length_bp: 825
variable_sites: 0
pi: NA
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.00
markerseek_score: 46.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	CGCTTCAACCGGGTTATTCT	ACGTGTCACAGTTAATTACTCCT	50.0	39.1	58.3	57.5	956 bp	1.0	54.2
rpl2_p2	ATCATTACGCTTCAACCGGG	ACGTGTCACAGTTAATTACTCCT	50.0	39.1	58.3	57.5	963 bp	1.0	53.6
rpl2_p3	ACGCTTCAACCGGGTTATTC	ACGTGTCACAGTTAATTACTCCT	50.0	39.1	58.6	57.5	957 bp	1.0	51.9
rpl2_p4	CGCTTCAACCGGGTTATTCT	ACGTGTCACAGTTAATTACTCC	50.0	40.9	58.3	56.1	956 bp	1.0	51.0
rpl2_p5	ATCATTACGCTTCAACCGGG	ACGTGTCACAGTTAATTACTCC	50.0	40.9	58.3	56.1	963 bp	1.0	50.4