Hotspot

ndhE-ndhG

SSC · 115160-115378 · 219 bp

Back to Kummerowia

Pi 0.055

MarkerSeek score 71.30

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 219
variable_sites: 12
pi: 0.055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	CACTCAAAACAAGTGCATGCT	TAGGTGCAATTGTTGTGGCT	42.9	45.0	57.9	58.0	279-287 bp	1.0	74.9
ndhE-ndhG_p2	CTCAAAACAAGTGCATGCTCA	TAGGTGCAATTGTTGTGGCT	42.9	45.0	57.6	58.0	277-285 bp	1.0	72.8
ndhE-ndhG_p3	ACTCAAAACAAGTGCATGCTC	TAGGTGCAATTGTTGTGGCT	42.9	45.0	57.6	58.0	278-286 bp	1.0	72.8
ndhE-ndhG_p4	TCAAAACAAGTGCATGCTCA	TAGGTGCAATTGTTGTGGCT	40.0	45.0	56.5	58.0	276-284 bp	1.0	71.3
ndhE-ndhG_p5	ACTCAAAACAAGTGCATGCT	TAGGTGCAATTGTTGTGGCT	40.0	45.0	56.4	58.0	278-286 bp	1.0	71.2