Hotspot

ndhD-psaC

SSC · 114181-114308 · 128 bp

Back to Kummerowia

Pi 0.0635

MarkerSeek score 76.90

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 128
variable_sites: 8
pi: 0.0635
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GCGGCTATTGGCAAAACTAC	AAACAACTCGAAGCATGGGT	50.0	45.0	57.8	58.0	211-213 bp	1.0	82.1
ndhD-psaC_p2	GCGGCTATTGGCAAAACTAC	GGCATGAAACAACTCGAAGC	50.0	50.0	57.8	58.0	217-219 bp	1.0	81.9
ndhD-psaC_p3	GAAAATTAAGGAACCCGCGG	AAACAACTCGAAGCATGGGT	50.0	45.0	57.4	58.0	227-229 bp	1.0	78.9
ndhD-psaC_p4	GCGGCTATTGGCAAAACTAC	GTAAGAGGTGTGAATCCGCC	50.0	55.0	57.8	58.3	274-276 bp	1.0	78.6
ndhD-psaC_p5	GCGGCTATTGGCAAAACTAC	TGTAAGAGGTGTGAATCCGC	50.0	50.0	57.8	57.6	275-277 bp	1.0	78.3