Hotspot

trnL-UAG-ccsA

SSC · 111316-111403 · 88 bp

Back to Kummerowia

Pi 0.0568
MarkerSeek score 72.50
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
88
variable_sites
5
pi
0.0568
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA ATGGATACGACCGAAAACGA 50.0 45.0 57.9 57.1 208-220 bp 1.0 73.3
trnL-UAG-ccsA_p2 TAGACACGCTGCTCTTAGGA TGGATACGACCGAAAACGAT 50.0 45.0 57.9 57.1 207-219 bp 1.0 73.3
trnL-UAG-ccsA_p3 AAATTGGTAGACACGCTGCT ATGGATACGACCGAAAACGA 45.0 45.0 57.8 57.1 215-227 bp 1.0 72.8
trnL-UAG-ccsA_p4 AAATTGGTAGACACGCTGCT TGGATACGACCGAAAACGAT 45.0 45.0 57.8 57.1 214-226 bp 1.0 72.8
trnL-UAG-ccsA_p5 GTAGACACGCTGCTCTTAGG TGGATACGACCGAAAACGAT 55.0 45.0 57.8 57.1 208-220 bp 1.0 72.7