Hotspot

ndhF-rpl32

SSC · 109939-110527 · 589 bp

Back to Kummerowia

Pi 0.0375

MarkerSeek score 68.40

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 589
variable_sites: 22
pi: 0.0375
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTTTGTTGCCGTCGGAAAAA	TGATATAGAAGTGCGCTTTT	40.0	35.0	57.4	52.7	724-729 bp	1.0	42.6
ndhF-rpl32_p2	TTTTGTTGCCGTCGGAAAAA	TTGATATAGAAGTGCGCTTT	40.0	35.0	57.4	52.7	725-730 bp	1.0	42.6
ndhF-rpl32_p3	TTTTGTTGCCGTCGGAAAAA	TTTGATATAGAAGTGCGCTT	40.0	35.0	57.4	52.7	726-731 bp	1.0	42.6
ndhF-rpl32_p4	TTTTGTTGCCGTCGGAAAAA	TTTTGATATAGAAGTGCGCT	40.0	35.0	57.4	52.7	727-732 bp	1.0	42.6
ndhF-rpl32_p5	TTTTTGTTGCCGTCGGAAAA	TGATATAGAAGTGCGCTTTT	40.0	35.0	57.4	52.7	725-730 bp	1.0	42.6