Hotspot

trnQ-UUG-psaI

LSC · 55637-59485 · 3849 bp

Back to Kummerowia

Pi 0.0215

MarkerSeek score 64.20

Primer available yes

Label trnQ-UUG-psaI

Feature Metadata

label_name: trnQ-UUG-psaI
feature_type: igs
region: LSC
length_bp: 3849
variable_sites: 79
pi: 0.0215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psaI_p1	CCCAATGAGCCGTTTATCGA	CGGTACTTTCAATTGGACCG	50.0	50.0	58.1	56.8	683-818 bp	1.0	75.9
trnQ-UUG-psaI_p2	TCCCAATGAGCCGTTTATCG	CGGTACTTTCAATTGGACCG	50.0	50.0	58.1	56.8	684-819 bp	1.0	75.9
trnQ-UUG-psaI_p3	CCCAATGAGCCGTTTATCGA	ACCGGTACTTTCAATTGGACC	50.0	47.6	58.1	58.2	685-820 bp	1.0	75.6
trnQ-UUG-psaI_p4	TCCCAATGAGCCGTTTATCG	ACCGGTACTTTCAATTGGACC	50.0	47.6	58.1	58.2	686-821 bp	1.0	75.6
trnQ-UUG-psaI_p5	CCCAATGAGCCGTTTATCGA	TGGATGTTTCTGACACCTACG	50.0	47.6	58.1	57.7	798-933 bp	1.0	74.9