Hotspot

trnG-UCC-trnS-GCU

LSC · 53746-54131 · 386 bp

Back to Kummerowia

Pi 0.043

MarkerSeek score 72.40

Primer available yes

Label trnG-UCC-trnS-GCU

Feature Metadata

label_name: trnG-UCC-trnS-GCU
feature_type: igs
region: LSC
length_bp: 386
variable_sites: 16
pi: 0.043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnS-GCU_p1	GGGGTCCATAGAACGAATCG	CAATCCGACGCTTTAGTCCA	55.0	50.0	57.8	58.0	467-478 bp	1.0	83.8
trnG-UCC-trnS-GCU_p2	GGGGTCCATAGAACGAATCG	AATCCGACGCTTTAGTCCAC	55.0	50.0	57.8	58.0	466-477 bp	1.0	83.8
trnG-UCC-trnS-GCU_p3	GGTCCATAGAACGAATCGCA	CAATCCGACGCTTTAGTCCA	50.0	50.0	57.8	58.0	465-476 bp	1.0	83.3
trnG-UCC-trnS-GCU_p4	GGTCCATAGAACGAATCGCA	AATCCGACGCTTTAGTCCAC	50.0	50.0	57.8	58.0	464-475 bp	1.0	83.3
trnG-UCC-trnS-GCU_p5	GGGGTCCATAGAACGAATCG	CAACGATCAGCAACGGAAAC	55.0	50.0	57.8	58.0	538-549 bp	1.0	82.8