Hotspot

trnH-GUG-psbA

LSC · 1645-1926 · 282 bp

Back to Kummerowia

Pi 0.0554

MarkerSeek score 85.40

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 282
variable_sites: 15
pi: 0.0554
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCCTCTAGATCTAGCTGCGA	50.0	55.0	57.9	57.8	346-356 bp	1.0	83.3
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TCCCTCTAGATCTAGCTGCG	50.0	55.0	57.9	57.8	347-357 bp	1.0	83.3
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	CCCTCTAGATCTAGCTGCGA	50.0	55.0	58.2	57.8	347-357 bp	1.0	83.1
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	TCCCTCTAGATCTAGCTGCG	50.0	55.0	58.2	57.8	348-358 bp	1.0	83.1
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	CCGAGCTAACCTTGGTATGG	50.0	55.0	57.9	57.8	397-407 bp	1.0	82.2