Hotspot

ycf91

Genome · 73507-73860 · 354 bp

Back to Kumanoa

Pi 0.186

MarkerSeek score 73.60

Primer available yes

Label ycf91

Feature Metadata

label_name: ycf91
feature_type: gene
region: Genome
length_bp: 354
variable_sites: 66
pi: 0.186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf91_p1	AGCATCTGACGCTCAAAACA	GGCTGCACCAGTAATACTCC	45.0	55.0	58.1	58.0	1393 bp	0.5	58.7
ycf91_p2	ACCGGAATCTGGCACTCTAT	GGCTGCACCAGTAATACTCC	50.0	55.0	58.2	58.0	1092 bp	0.5	58.0
ycf91_p3	TGGCACTCTATCCACTGAGT	GGCTGCACCAGTAATACTCC	50.0	55.0	57.8	58.0	1083 bp	0.5	57.7
ycf91_p4	AGCATCTGACGCTCAAAACA	ACTCCAATCTCCTGTTTGGC	45.0	50.0	58.1	57.8	1378 bp	0.5	57.4
ycf91_p5	AGCATCTGACGCTCAAAACA	TGTATCCTGTAGGTTGGGCT	45.0	50.0	58.1	57.7	1736 bp	0.5	57.0