Hotspot

ycf53

Genome · 113899-114648 · 750 bp

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Pi 0.138

MarkerSeek score 79.40

Primer available yes

Label ycf53

Feature Metadata

label_name: ycf53
feature_type: gene
region: Genome
length_bp: 750
variable_sites: 103
pi: 0.138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf53_p1	CACGATGCAGCACATTTGTT	AGTTTGGCAGCACTGGTTAA	45.0	45.0	57.9	57.9	1227 bp	0.5	59.0
ycf53_p2	CACGATGCAGCACATTTGTT	TTAGTTTGGCAGCACTGGTT	45.0	45.0	57.9	57.9	1229 bp	0.5	59.0
ycf53_p3	CTCACGATGCAGCACATTTG	AGTTTGGCAGCACTGGTTAA	50.0	45.0	58.1	57.9	1229 bp	0.5	58.6
ycf53_p4	CTCACGATGCAGCACATTTG	TTAGTTTGGCAGCACTGGTT	50.0	45.0	58.1	57.9	1231 bp	0.5	58.6
ycf53_p5	GGACCTGGAGATCCTGAAGT	AGTTTGGCAGCACTGGTTAA	55.0	45.0	58.1	57.9	1584 bp	0.5	58.5