Hotspot

orf466

Genome · 12913-14310 · 1398 bp

Back to Kumanoa

Pi 0.167

MarkerSeek score 79.50

Primer available yes

Label orf466

Feature Metadata

label_name: orf466
feature_type: gene
region: Genome
length_bp: 1398
variable_sites: 233
pi: 0.167
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf466_p1	CAGGACCAGCCCATATAGGA	CCAAGCTCGAGGCAGTAATT	55.0	50.0	58.0	58.0	2621 bp	0.5	59.5
orf466_p2	CAGGACCAGCCCATATAGGA	TTAAACCAAGCTCGAGGCAG	55.0	50.0	58.0	58.2	2626 bp	0.5	58.2
orf466_p3	ATGCAGGACCAGCCCATATA	CCAAGCTCGAGGCAGTAATT	50.0	50.0	58.3	58.0	2624 bp	0.5	57.7
orf466_p4	TATGCAGGACCAGCCCATAT	CCAAGCTCGAGGCAGTAATT	50.0	50.0	58.3	58.0	2625 bp	0.5	57.7
orf466_p5	CAGGACCAGCCCATATAGGA	CCAGAAGTACATCCGCTGTT	55.0	50.0	58.0	57.6	2337 bp	0.5	56.7