Hotspot

orf172

Genome · 138067-138576 · 510 bp

Back to Kumanoa

Pi 0.21

MarkerSeek score 76.90

Primer available yes

Label orf172

Feature Metadata

label_name: orf172
feature_type: gene
region: Genome
length_bp: 510
variable_sites: 107
pi: 0.21
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf172_p1	GAACCAGTCCGTTAACGTGT	TAAAGTGGGAGCAGCAACTG	50.0	50.0	58.1	58.1	1284 bp	0.5	58.0
orf172_p2	GAACCAGTCCGTTAACGTGT	GCCAAATTGTCAGGAGGAGT	50.0	50.0	58.1	57.8	1313 bp	0.5	57.2
orf172_p3	GAACCAGTCCGTTAACGTGT	AGGACAGGCTAGACGTGTTA	50.0	50.0	58.1	57.8	1467 bp	0.5	57.2
orf172_p4	GAACCAGTCCGTTAACGTGT	TAGGACAGGCTAGACGTGTT	50.0	50.0	58.1	57.8	1468 bp	0.5	57.2
orf172_p5	GAACCAGTCCGTTAACGTGT	TGTTAGGACAGGCTAGACGT	50.0	50.0	58.1	57.8	1471 bp	0.5	57.2