Hotspot

ORF198

Genome · 61522-62733 · 1212 bp

Back to Kumanoa

Pi 0.192

MarkerSeek score 79.20

Primer available yes

Label ORF198

Feature Metadata

label_name: ORF198
feature_type: gene
region: Genome
length_bp: 1212
variable_sites: 233
pi: 0.192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF198_p1	TCAGTAATGCATCTGTGGCC	CGTCAAGGTTTTCCAGGGAA	50.0	50.0	58.0	58.0	2401 bp	0.5	59.5
ORF198_p2	TCAGTAATGCATCTGTGGCC	TTCGTCAAGGTTTTCCAGGG	50.0	50.0	58.0	58.0	2403 bp	0.5	59.5
ORF198_p3	TCAGTAATGCATCTGTGGCC	TAACTCGCGTAGGAGAACCA	50.0	50.0	58.0	58.2	2256 bp	0.5	58.3
ORF198_p4	TCAGTAATGCATCTGTGGCC	TCGCGTAGGAGAACCACTTA	50.0	50.0	58.0	58.2	2252 bp	0.5	58.3
ORF198_p5	TCAGTAATGCATCTGTGGCC	CCTTTTCAGGATGGCGGATT	50.0	50.0	58.0	58.2	1974 bp	0.5	57.8