Hotspot

ycf33-argB

Genome · 160822-161161 · 340 bp

Back to Kumanoa

Pi 0.275

MarkerSeek score 76.90

Primer available yes

Label ycf33-argB

Feature Metadata

label_name: ycf33-argB
feature_type: igs
region: Genome
length_bp: 340
variable_sites: 83
pi: 0.275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf33-argB_p1	TGCAGTAGCTATTCCCGAGA	AGCAGCATTGAACTTTGGGA	50.0	45.0	57.9	58.0	1721 bp	0.5	59.5
ycf33-argB_p2	TGCAGTAGCTATTCCCGAGA	TCAAGCGAAGGCAGAAGTTT	50.0	45.0	57.9	58.0	1038 bp	0.5	59.2
ycf33-argB_p3	TGCAGTAGCTATTCCCGAGA	CAAGCGAAGGCAGAAGTTTG	50.0	50.0	57.9	58.0	1037 bp	0.5	59.1
ycf33-argB_p4	TGCAGTAGCTATTCCCGAGA	CCAGCCAAAACCATTTCGAC	50.0	50.0	57.9	57.9	1287 bp	0.5	58.9
ycf33-argB_p5	GTGCAGTAGCTATTCCCGAG	AGCAGCATTGAACTTTGGGA	55.0	45.0	57.9	58.0	1722 bp	0.5	58.9