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BIZ62_gr001-ycf27

Genome · 132172-132733 · 562 bp

Back to Kumanoa

Pi 0.31

MarkerSeek score 82.50

Primer available yes

Label BIZ62_gr001-ycf27

Feature Metadata

label_name: BIZ62_gr001-ycf27
feature_type: igs
region: Genome
length_bp: 562
variable_sites: 171
pi: 0.31
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
BIZ62_gr001-ycf27_p1	CGTCCTCTCAAACCAGCTAC	CTCCATCAGTGGCGCTTATT	55.0	50.0	58.0	58.0	942-987 bp	1.0	88.5
BIZ62_gr001-ycf27_p2	TAATGGCCTAGTAGAGCGCT	CTCCATCAGTGGCGCTTATT	50.0	50.0	58.0	58.0	1363-1408 bp	1.0	88.2
BIZ62_gr001-ycf27_p3	CGTCCTCTCAAACCAGCTAC	GCTCGTGAAAAAGCTTCACC	55.0	50.0	58.0	58.0	1375 bp	0.5	59.7
BIZ62_gr001-ycf27_p4	TAATGGCCTAGTAGAGCGCT	GCTCGTGAAAAAGCTTCACC	50.0	50.0	58.0	58.0	1796 bp	0.5	59.7
BIZ62_gr001-ycf27_p5	CATTGCGGAAAATTCCCCAC	GCTCGTGAAAAAGCTTCACC	50.0	50.0	58.0	58.0	1446 bp	0.5	59.6