Hotspot

BIZ62_gr002-BIZ62_gt018

Genome · 130439-130489 · 51 bp

Back to Kumanoa

Pi 0.214

MarkerSeek score 80.80

Primer available yes

Label BIZ62_gr002-BIZ62_gt018

Feature Metadata

label_name: BIZ62_gr002-BIZ62_gt018
feature_type: igs
region: Genome
length_bp: 51
variable_sites: 9
pi: 0.214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
BIZ62_gr002-BIZ62_gt018_p1	ATTTCGTAGGTAACCACGCC	GTTCGAGTCCGCTTATCTCC	50.0	55.0	58.0	57.9	135-144 bp	1.0	88.1
BIZ62_gr002-BIZ62_gt018_p2	ATTTCGTAGGTAACCACGCC	GGTTCGAGTCCGCTTATCTC	50.0	55.0	58.0	57.9	136-145 bp	1.0	88.0
BIZ62_gr002-BIZ62_gt018_p3	TCATCGCTTCTGAATGCCAA	CCAACCAAAGGGGGTATAGC	45.0	55.0	57.9	57.9	174-183 bp	1.0	86.6
BIZ62_gr002-BIZ62_gt018_p4	ATTTCGTAGGTAACCACGCC	CCAACCAAAGGGGGTATAGC	50.0	55.0	58.0	57.9	196-205 bp	1.0	86.5
BIZ62_gr002-BIZ62_gt018_p5	AAGCTTGTTTCCAGCTACCC	CCAACCAAAGGGGGTATAGC	50.0	55.0	58.1	57.9	221-230 bp	1.0	85.1