Hotspot

BIZ62_gt017-dsbD

Genome · 122408-122670 · 263 bp

Back to Kumanoa

Pi 0.308

MarkerSeek score 74.20

Primer available yes

Label BIZ62_gt017-dsbD

Feature Metadata

label_name: BIZ62_gt017-dsbD
feature_type: igs
region: Genome
length_bp: 263
variable_sites: 80
pi: 0.308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
BIZ62_gt017-dsbD_p1	CTACGGCCAATCGGTTAACA	TCCGCCGGTAAAAGGAAAAA	50.0	45.0	58.0	58.0	1206 bp	0.5	59.7
BIZ62_gt017-dsbD_p2	TACGGCCAATCGGTTAACAG	TCCGCCGGTAAAAGGAAAAA	50.0	45.0	58.0	58.0	1205 bp	0.5	59.7
BIZ62_gt017-dsbD_p3	CAGGTTCACGTATGCCATCA	TCCGCCGGTAAAAGGAAAAA	50.0	45.0	58.0	58.0	1635 bp	0.5	59.6
BIZ62_gt017-dsbD_p4	ACAGGTTCACGTATGCCATC	TCCGCCGGTAAAAGGAAAAA	50.0	45.0	58.0	58.0	1636 bp	0.5	59.6
BIZ62_gt017-dsbD_p5	TAGCGGATGTGGTGGAATTG	TCCGCCGGTAAAAGGAAAAA	50.0	45.0	58.0	58.0	1023 bp	0.5	59.5