Hotspot

psaM-chlL

Genome · 102630-103295 · 666 bp

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Pi 0.243

MarkerSeek score 83.90

Primer available yes

Label psaM-chlL

Feature Metadata

label_name: psaM-chlL
feature_type: igs
region: Genome
length_bp: 666
variable_sites: 154
pi: 0.243
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaM-chlL_p1	AAGGAAAAACCGGACGAACA	AGCTGCAAAACCACCACATA	45.0	45.0	58.0	58.0	1385-1410 bp	1.0	90.2
psaM-chlL_p2	AGGAAAAACCGGACGAACAA	AGCTGCAAAACCACCACATA	45.0	45.0	58.0	58.0	1384-1409 bp	1.0	90.2
psaM-chlL_p3	CTTCTGTTGCTCCCAAAGGT	AGCTGCAAAACCACCACATA	50.0	45.0	58.0	58.0	1701 bp	0.5	59.8
psaM-chlL_p4	CTTCTGTTGCTCCCAAAGGT	CAGCTATTCGATTTGCTGCG	50.0	50.0	58.0	58.0	1780 bp	0.5	59.8
psaM-chlL_p5	CTTCTGTTGCTCCCAAAGGT	AACGTCTTCTGGCCAAACAT	50.0	45.0	58.0	58.0	1518 bp	0.5	59.7