Hotspot

gltB-rpoZ

Genome · 86292-86817 · 526 bp

Back to Kumanoa

Pi 0.233

MarkerSeek score 81.10

Primer available yes

Label gltB-rpoZ

Feature Metadata

label_name: gltB-rpoZ
feature_type: igs
region: Genome
length_bp: 526
variable_sites: 118
pi: 0.233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
gltB-rpoZ_p1	CACGATCTGCACTACAAGCT	ATGCATTTCCACCAGGTTGT	50.0	45.0	58.0	58.0	1339-1372 bp	1.0	89.1
gltB-rpoZ_p2	TCACGATCTGCACTACAAGC	ATGCATTTCCACCAGGTTGT	50.0	45.0	58.0	58.0	1340-1373 bp	1.0	89.0
gltB-rpoZ_p3	CACGATCTGCACTACAAGCT	TCAGCCTGAGCAACAACTTT	50.0	45.0	58.0	57.9	1157-1190 bp	1.0	88.9
gltB-rpoZ_p4	TCACGATCTGCACTACAAGC	TCAGCCTGAGCAACAACTTT	50.0	45.0	58.0	57.9	1158-1191 bp	1.0	88.9
gltB-rpoZ_p5	ATGACCGGAACTAACCTCCA	ATGCATTTCCACCAGGTTGT	50.0	45.0	58.1	58.0	1553 bp	0.5	59.5